Best practices for optimizing Cell Line Development processes
In this article, we spoke with Dr Jon Dempsey about his thoughts regarding best practices for optimizing CLD processes, Founding Partner of Pathway Biopharma
Cell line development (CLD) follows a little changed process from that used in the early days of biopharmaceutical development. The steps to create a stable, manufacturing cell line include host cell line selection, engineering of the expression vector, transfection, cloning and cell expansion, as well as various screening steps to select the clone with the highest cell viability, growth, expression level, stability and product quality. However, within this process, there are many different strategies that companies can adopt to optimize their workflow. Download to continue reading…
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